RAPID SEPARATION OF GONADOTROPIN-RELEASING-HORMONE MOLECULAR-FORMS BYISOCRATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON AN ION-EXCHANGE COLUMN

The purpose of the present work was to develop a chromatographic syste m for the separation of five molecular forms of the gonadotropin-relea sing hormone (GnRH); mammalian GnRH (mGnRH) (LHRH), salmon GnRH (sGnRH ), chicken I GnRH (cIGnRH), chicken II GnRH (cIIGnRH) and lamprey GnRH I (IGnRH-I). By using an ion-exchange HPLC column and isocratic eluti on, it was possible to separate properly the five peptides in approxim ately 20 min. The utility of the system in determining the GnRHs forms present in the brain of two species of vertebrates was examined.