DETERMINATION OF SCOPOLAMINE IN HUMAN SERUM BY GAS-CHROMATOGRAPHY ION-TRAP TANDEM MASS-SPECTROMETRY

The objective of this study was to develop a very sensitive and select ive method for the determination of scopolamine in serum with a rapid and simple sample preparation. A capillary column gas chromatographic- ion trap tandem mass spectrometric technique has been applied. Scopola mine and the internal standard mexiletine were extracted from serum sa mples and cleaned up by using a single step liquid-liquid extraction. Derivatization was carried out using ,2,2-trifluoro-N-methyl-N-trimeth ylsilylacetamide. The mass spectrometer was operated with positive ion s in the selected reaction mode with chemical ionisation using methane . The sum of peak height of two daughter ions was used for quantificat ion. The detection limit was 50 pg/ml in serum.