Mp. Turcich et al., SEQUENCE AND EXPRESSION OF A MICROSPORE CDNA CLONE WITH HOMOLOGY TO ARIBOSOMAL-PROTEIN, Acta Societatis Botanicorum Poloniae, 65(1-2), 1996, pp. 61-63
A cDNA library was made to RNA from corn anthers containing developing
pollen at the uninucleate microspore stage. A randomly selected clone
from this library which contained an insert (531 bp) was isolated and
sequenced. An open reading frame of 330 bp was located. Computer alig
nments of the putative amino acid sequence with sequences from GenBank
and the SwissProt protein databases indicated homology to L12, an aci
dic ribosomal protein. RNA blot analysis showed highest levels of this
mRNA in mature pollen. The significance of this observation in light
of the known biochemistry of ribosome synthesis in developing pollen i
s discussed.