CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A VANADIUM-DEPENDENT PEROXIDASE FROM ASCOPHYLLUM-NODOSUM

Authors
WEYAND M HECHT HJ VILTER H SCHOMBURG D
Citation
M. Weyand et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A VANADIUM-DEPENDENT PEROXIDASE FROM ASCOPHYLLUM-NODOSUM, Acta crystallographica. Section D, Biological crystallography, 52, 1996, pp. 864-865
Citations number
31
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
52
Year of publication
1996
Part
4
Pages
864 - 865
Database
ISI
SICI code
0907-4449(1996)52:<864:CAPAOA>2.0.ZU;2-O
Abstract
Peroxidase from the brown alga Ascophyllum nodosum, a nonheme vanadium -dependent haloperoxidase, has been purified to homogeneity and crysta llized from ammonium sulfate solutions in a form suitable for X-ray di ffraction analysis. The crystals have been grown by the vapour-diffusi on technique using the sitting-drop method. X-ray diffraction studies show that the crystals belong to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2 with a = b = 114.3 and c = 276.0 Angstrom. The crystals diffract to at least 2.4 Angstrom resolution.