CRYSTALS OF THE CHEMICALLY SYNTHESIZED ACCEPTOR STEM OF TRNA(ALA) FROM ESCHERICHIA-COLI DIFFRACTING TO HIGH-RESOLUTION

Authors
OTT G DORFLER S SPRINZL M MULLER U HEINEMANN U
Citation
G. Ott et al., CRYSTALS OF THE CHEMICALLY SYNTHESIZED ACCEPTOR STEM OF TRNA(ALA) FROM ESCHERICHIA-COLI DIFFRACTING TO HIGH-RESOLUTION, Acta crystallographica. Section D, Biological crystallography, 52, 1996, pp. 871-873
Citations number
25
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
52
Year of publication
1996
Part
4
Pages
871 - 873
Database
ISI
SICI code
0907-4449(1996)52:<871:COTCSA>2.0.ZU;2-5
Abstract
The acceptor stem of tRNA(Ala) from E. coli has been chemically synthe sized and crystallized. This duplex contains a G . U base pair in posi tion 3-70, which is the main identity element for alanyl-tRNA syntheta se from E. coil. The crystals are stable in the X-ray beam for a long period of time and diffract to 1.7 Angstrom resolution. The monoclinic crystals reveal a CZ space group with a = 35.0, b = 47.5, c = 26.2 An gstrom, beta = 102.3 degrees and one acceptor stem per asymmetric unit .