C. Rang et al., INFLUENCE OF THE 20-KDA PROTEIN FROM BACILLUS-THURINGIENSIS SSP ISRAELENSIS ON THE RATE OF PRODUCTION OF TRUNCATED CRY1C PROTEINS, FEMS microbiology letters, 141(2-3), 1996, pp. 261-264
The potential role of a molecular chaperone on the rate of production
of extensively altered Bacillus thuringiensis Cry1C proteins was inves
tigated. Analysis of the proteins produced by the recombinant B. thuri
ngiensis strains showed that the truncated proteins were produced at a
low rate. Expression of the 20-kDa protein gene from B. thuringiensis
ssp, israelensis in tandem with the truncated-cry1C genes led to the
production of a greater amount of proteins. The formation of inclusion
bodies, however, did not occur even when the 20-kDa protein gene was
expressed.