Dk. Jaiswal et al., SYNTHESIS OF HIGH SPECIFIC ACTIVITY TRITIUM-LABELED [2-H-3]-ADENOSINE-5'-TRIPHOSPHATE, Journal of labelled compounds & radiopharmaceuticals, 38(8), 1996, pp. 743-752
Citations number
16
Categorie Soggetti
Chemistry Analytical","Pharmacology & Pharmacy","Biochemical Research Methods
A procedure for high level tritium labelling at the C2-H position of a
denosine 5'-triphosphate ([2-H-3]-ATP, 1), based on the tritiodehaloge
nation reaction of 2-bromoadenosine 5'-triphosphate (2) has been elabo
rated. This precursor was prepared in a six-step synthesis from guanos
ine. The tritiodehalogenation of (2) for three hours over palladium ox
ide in phosphate buffer yielded tritium labelled ATP with high specifi
c activity, in good chemical yield.