LONG-TERM EXPRESSION OF FOS-RELATED ANTIGEN AND TRANSIENT EXPRESSION OF DELTA-FOSB ASSOCIATED WITH SEIZURES IN THE RAT HIPPOCAMPUS AND STRIATUM

Citation
Gy. Bing et al., LONG-TERM EXPRESSION OF FOS-RELATED ANTIGEN AND TRANSIENT EXPRESSION OF DELTA-FOSB ASSOCIATED WITH SEIZURES IN THE RAT HIPPOCAMPUS AND STRIATUM, Journal of neurochemistry, 68(1), 1997, pp. 272-279
Citations number
44
Categorie Soggetti
Biology,Neurosciences
Journal title
ISSN journal
00223042
Volume
68
Issue
1
Year of publication
1997
Pages
272 - 279
Database
ISI
SICI code
0022-3042(1997)68:1<272:LEOFAA>2.0.ZU;2-B
Abstract
Systemic administration of kainic acid (KA), an analogue of glutamic a cid, causes limbic seizures and pathophysiological changes in adult ra ts that are very similar to human temporal lobe epilepsy. One of the e arliest changes in gene expression after treatment with KA is the indu ction of immediate-early genes. The fos and jun families are frequentl y studied immediate-early genes that are induced by KA. Several groups , including ours, have recently reported that a 35-kDa Fos-related ant igen (FRA) is induced for a protracted time by various stimuli. It has been suggested that this FRA is Delta FosB, which has a molecular mas s of similar to 35 kDa, The present study characterizes the long-term expression of FRA and Delta FosB after systemic treatment with KA. Imm unocytochemistry and western blot analysis using an antibody that cros s-reacts with all known FRAs showed that a 35-kDa FRA was induced at h igh levels in both the hippocampus and striatum for up to 1 month by K A, A semiquantitative PCR analysis showed that Delta FosB was induced by KA, but its expression lasted for only 6 h. This result was also ve rified by northern blot analysis, These results suggested that the 35- kDa FRA with long-term elevated levels seen with western blot analysis and immunocytochemistry is a new species of the FRA and not Delta Fos B. The long-term expression of FRA in both the hippocampus and striatu m may be associated with the pathophysiological changes after KA admin istration.