INCREASED MACROMOLECULAR RESONANCES IN THE RAT CEREBRAL-CORTEX DURINGSEVERE ENERGY FAILURE AS DETECTED BY H-1 NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY

Authors
HAKUMAKI JM GROHN OHJ PIRTTILA TRM KAUPPINEN RA
Citation
Jm. Hakumaki et al., INCREASED MACROMOLECULAR RESONANCES IN THE RAT CEREBRAL-CORTEX DURINGSEVERE ENERGY FAILURE AS DETECTED BY H-1 NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY, Neuroscience letters, 212(3), 1996, pp. 151-154
Citations number
23
Categorie Soggetti
Neurosciences
Journal title
Neuroscience letters → ACNP
ISSN journal
03043940
Volume
212
Issue
3
Year of publication
1996
Pages
151 - 154
Database
ISI
SICI code
0304-3940(1996)212:3<151:IMRITR>2.0.ZU;2-C
Abstract
Changes in cerebral macromolecular H-1 nuclear magnetic resonance (NMR ) spectrum were studied in cortical brain slices in vitro. Aglycaemic hypoxia irreversibly increased various short T-2 spectral components a t 1.8-0.8 ppm in concordance with energy loss and independent of T-1 a nd T-2 relaxation effects. Removal of external calcium (Ca-e(2+)) slig htly attenuated the effect. The results suggest NMR-visible reorganisa tion of intracellular proteins due to hypoxic insult, and show that it may be possible to monitor early cytoplasmic changes due to brain ene rgy depletion by NMR spectroscopy.