Bt. Podsiadly et al., PREGNANCY RESULTING FROM INTRACYTOPLASMIC INJECTION OF CRYOPRESERVED SPERMATOZOA RECOVERED FROM TESTICULAR BIOPSY, Human reproduction, 11(6), 1996, pp. 1306-1308
We report a clinical pregnancy occurring in a 31 year old patient foll
owing intracytoplasmic sperm injection (ICSI) of cryopreserved spermat
ozoa obtained from a testicular biopsy. This was the couple's second a
ttempt at an ovarian stimulated cycle resulting in the collection of 1
7 metaphase II ova which were all injected with progressively motile s
permatozoa. A fertilization rate of 58% and a cleavage rate of 90% wer
e achieved. This report is our first case of ICSI using cryopreserved
testicular spermatozoa which resulted in normal fertilization, embryo
development and an on-going singleton pregnancy.