M. Satake et Mc. Liberman, MORPHOLOGICAL SUBCLASSES OF LATERAL OLIVOCOCHLEAR TERMINALS - ULTRASTRUCTURAL ANALYSIS OF INNER SPIRAL BUNDLE IN CAT AND GUINEA-PIG, Journal of comparative neurology, 371(4), 1996, pp. 621-632
The lateral olivocochlear efferent pathway terminates in vesicle-fille
d swellings in the inner spiral bundles under inner hair cells (IHCs)
and has been suggested to include at least two chemically distinct sub
classes (see, e.g., Vetter et al. [1991] Synapse 7:21-43). In the pres
ent study, the ultrastructure and peripheral targets of vesicle-filled
swellings in the IHC area of the cat and guinea pig cochleas were qua
ntitatively analyzed to determine 1) whether morphological subclasses
could be defined based on swelling size or on the density, size, or sh
ape of clear and dense-cored vesicles and 2) whether swellings with di
fferent postsynaptic targets differed morphologically. In both cat and
guinea pig, all swellings contained large, round, clear vesicles and
a variable number of dense-core vesicles. Although evidence of clear-c
ut subclasses was not compelling, the smallest swellings tended to be
rich in dense-core and poor in clear vesicles and rarely formed synapt
ic contacts. Most of the larger swellings, which tended to contain few
dense-core vesicles and a rich complement of clear round vesicles, fo
rmed synapses with radial afferent fibers. However, there were no morp
hological differences between swellings contacting afferents originati
ng on the modiolar vs. pillar sides of the IHC (the source of afferent
s with low and high spontaneous discharge rates, respectively). We con
clude that 1) if distinct gamma-amino butyric acid (GABA)ergic and cho
linergic subclasses of lateral olivocochlear (LOG) fibers exist, then
the vesicle morphology of their terminals does not differ as it does i
n the central nervous system and that 2) if peptide neurotransmitters,
such as calcitonin gene-related peptide and enkephalins, are packaged
in dense-core vesicles, then the LOC terminals synapsing with IHC aff
erent fibers are not particularly rich in these peptides. (C) 1996 Wil
ey-Liss, Inc.