LINEAR CORRELATION BETWEEN BACTERIAL OVEREXPRESSION OF RECOMBINANT PEPTIDES AND CELL LIGHT SCATTER

Authors
LAVERGNEMAZEAU F MAFTAH A CENATIEMPO Y JULIEN R
Citation
F. Lavergnemazeau et al., LINEAR CORRELATION BETWEEN BACTERIAL OVEREXPRESSION OF RECOMBINANT PEPTIDES AND CELL LIGHT SCATTER, Applied and environmental microbiology, 62(8), 1996, pp. 3042-3046
Citations number
13
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
62
Issue
8
Year of publication
1996
Pages
3042 - 3046
Database
ISI
SICI code
0099-2240(1996)62:8<3042:LCBBOO>2.0.ZU;2-A
Abstract
Fusion of multiple copies of a test peptide leads to insoluble inclusi on bodies. Their presence within bacteria increases either forward-ang le light scattering or, to lesser extent, right-angle light scattering . A linear correlation has been established between cell forward-angle light scattering and the level of overexpression of atrial natriureti c peptide. The correlation is valid only for unlysed cells and is prot ein product specific.