P. Bauerkreisel et al., QUANTIFICATION OF DEHALOSPIRILLUM-MULTIVORANS IN MIXED-CULTURE BIOFILMS WITH AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY, Applied and environmental microbiology, 62(8), 1996, pp. 3050-3052
A fast, highly selective and sensitive method to quantify specific bio
masses in mixed-culture biofilms is described. It consists of detachme
nt of a biofilm from its support material, resolution of the detached
biofilm flocs in order to separate the enclosed cells and antigens, an
d quantification of specific biomass by an enzyme-linked immunosorbent
assay.