BETA-METHYLTHIO-ASPARTIC ACID - IDENTIFICATION OF A NOVEL POSTTRANSLATIONAL MODIFICATION IN RIBOSOMAL-PROTEIN S12 FROM ESCHERICHIA-COLI

Authors
KOWALAK JA WALSH KA
Citation
Ja. Kowalak et Ka. Walsh, BETA-METHYLTHIO-ASPARTIC ACID - IDENTIFICATION OF A NOVEL POSTTRANSLATIONAL MODIFICATION IN RIBOSOMAL-PROTEIN S12 FROM ESCHERICHIA-COLI, Protein science, 5(8), 1996, pp. 1625-1632
Citations number
32
Categorie Soggetti
Biology
Journal title
Protein science → ACNP
ISSN journal
09618368
Volume
5
Issue
8
Year of publication
1996
Pages
1625 - 1632
Database
ISI
SICI code
0961-8368(1996)5:8<1625:BA-IOA>2.0.ZU;2-6
Abstract
Utilizing microscale chemical derivatization reactions and matrix-assi sted laser desorption/ionization time-of-flight mass spectrometry, we have identified a novel posttranslational modification of aspartic aci d, beta-methylthio-aspartic acid. The modified residue is located at p osition 88 in ribosomal protein S12 from Escherichia coli, a phylogene tically conserved protein that has been implicated in maintaining tran slational accuracy of the ribosome.