N-(2-FERROCENE-ETHYL)MALEIMIDE - A NEW ELECTROACTIVE SULFHYDRYL-SPECIFIC REAGENT FOR CYSTEINE-CONTAINING PEPTIDES AND PROTEINS

Authors
DIGLERIA K HILL HAO WONG LL
Citation
K. Digleria et al., N-(2-FERROCENE-ETHYL)MALEIMIDE - A NEW ELECTROACTIVE SULFHYDRYL-SPECIFIC REAGENT FOR CYSTEINE-CONTAINING PEPTIDES AND PROTEINS, FEBS letters, 390(2), 1996, pp. 142-144
Citations number
16
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
390
Issue
2
Year of publication
1996
Pages
142 - 144
Database
ISI
SICI code
0014-5793(1996)390:2<142:N-ANES>2.0.ZU;2-N
Abstract
We report the synthesis and application of a specific electroactive la bel, N-(2-ferrocene-ethyl)maleimide, which provides nem redox properti es to organic compounds and proteins possessing sulphydryl groups, Its reaction conditions with the cysteine-containing peptide, glutathione , and a terminal monooxygenase enzyme, cytochrome P450(cam) are presen ted. The labelled peptide and enzyme acquired reversible electrochemic al properties due to the attached ferrocene moiety.