POSITRON EMISSION TOMOGRAPHY FOR QUANTITATIVE-DETERMINATION OF GLUCOSE-METABOLISM IN NORMAL AND ISCHEMIC BRAINS IN RATS - AN INSOLUBLE PROBLEM BY THE HARDERIAN GLANDS
Y. Kuge et al., POSITRON EMISSION TOMOGRAPHY FOR QUANTITATIVE-DETERMINATION OF GLUCOSE-METABOLISM IN NORMAL AND ISCHEMIC BRAINS IN RATS - AN INSOLUBLE PROBLEM BY THE HARDERIAN GLANDS, Journal of cerebral blood flow and metabolism, 17(1), 1997, pp. 116-120
To examine the reliability of quantitative positron emission tomograph
y studies in the rat (Rat-PET), we assessed the influence of radioacti
vity accumulated in the Harderian glands on PET CMR(glc) determination
. We measured CMR(glc) by PET and ex vivo dissection methods by using
2-[F-18]fluoro-2-deoxy-D-glucose in rats with and without focal brain
ischemia. The CMR(glc) values obtained by PET, after correcting with r
ecovery coefficients, were higher than those measured by the ex vivo m
ethod at rostral slices, and reduction of the CMR(glc) in the ischemic
brain was not demonstrated by PET in the frontal cortex, The radioact
ivity accumulated in the Harderian glands prevents the quantitative de
termination of CMR(glc) using Rat-PET.