APPLICATION OF THIAMINE AS A FLUOROGENIC SUBSTRATE IN THE DETERMINATION OF HYDROGEN-PEROXIDE BASED ON THE CATALYTIC EFFECT OF HEMIN

A rapid and sensitive fluorimetric method has been developed for the d etermination of trace hydrogen peroxide (0 - 5.0 x 10(-6) mol/l) and, indirectly, glucose (3.0 x 10(-7) - 3.5 x 10(-5) mol/l), based on the catalytic effect of hemin on the thiamine, a new fluorogenic substrate , and hydrogen peroxide system at pH=8.0. Using the fixed time method, the detection limits of hydrogen peroxide and glucose are 3.0 x 10(-8 ) mol/l and 3.0 x 10(-7) mol/l, respectively. This method is simple, i nexpensive and has been applied successfully to the determination of g lucose in human sera with relative standard deviation of 2.4 - 3.1%.