STUDY ON THE ADSORPTIVE BEHAVIOR OF HESPERIDIN AND ITS ADSORPTIVE STRIPPING VOLTAMMETRY

A sensitive adsorptive stripping voltammetric method for measurement o f hesperidin in 0.1 M NaAc-HAc solution was described. The method was based on the adsorptive accumulation of the drug at a hanging mercury drop electrode, followed by linear sweep voltammetry. The response was evaluated with respect to preconcentration time, pH effect and concen tration of HAc-NaAc buffer solution. The peak potential was -1.01V (vs .Ag/AgCl). The peak current was directly proportional to the concentra tion of hesperidin with a detection limit of 3.0x10(-7) M at an accumu lation time of 2 min. The reduction process is irreversible and the wa ve shows adsorptive characteristics. Hesperidin, a flavanone glucoside , is a main composition in orange peel and is frequently seen as a com ponent in pharmaceutical preparations. It is significant to study the electrochemical behaviour of hesperidin, especially its adsorptive cha racteristics and its determination. It is known for its affect of lowe ring cholesterol and anti-viral property and its structure is shown in Figure 1. The determination of hesperidin has been carried out by flu orimetric analysis(1), ultra-violet spectrometry and coulometric titra tion(2). However, the electrochemical behaviour of hesperidin and its determination have not been reported so far. In this work the adsorpti ve characteristics of hesperidin and a new adsorptive stripping voltam metric method for the trace determination of hesperidin is proposed, T he detection limit is 3.0x10(-7) M. This method is simple, fast and se nsitive.