COMPLIMENTARY ROLE OF MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN THE SEPARATION OF PLANT PHENOLICS

A comparison was made between the efficiency of micellar electrokineti c capillary chromatography (MECC) and reversed phase high performance liquid chromatography (RP-HPLC) to separate a mixture of plant phenoli cs. Of a range of buffers 6 mM borate/10 mM phosphate/100 mM SDS at pH 8.5 was the most effective in separating a complex mixture of phenoli cs using MECC. Using this buffer the elution order and resolution was different from that obtained by HPLC using a reversed phase C18 column . These results illustrate how MECC and RP-HPLC are complimentary when examining complex mixtures such as those obtained from plant extracts . MECC using these conditions was applied to the examination of phenol ics from leaf tissue of Eucalyptus marginata (jarrah).