Enzymes usually cannot withstand the high-temperature curing associate
d with the thick-film fabrication process and require a separate immob
ilization step in connection with the production of single use biosens
ors, We report on the development of sol-gel-derived enzyme-containing
carbon inks that display compatibility with the screen-printing proce
ss, Such coupling of sol-gel and thick-film technologies offers a one-
step fabrication of disposable enzyme electrodes, as it obviates the n
eed for thermal curing. The enzyme-containing sol-gel carbon ink, prep
ared by dispersing the biocatalyst, along with the graphite powder and
a binder, within the sol-gel precursors, is cured very rapidly (10 mi
n) at low temperature (4 degrees C). The influence of the ink preparat
ion conditions is explored, and the sensor performance is evaluated in
connection with the incorporation of glucose oxidase or horseradish p
eroxidase, The resulting strips are stable for at least 3 months, Such
sol-gel-derived carbon inks should serve as hosts for other heat-sens
itive biomaterials in connection with the microfabrication of various
thick-film biosensors.