HRGC FID/NPD AND HRGC/MSD STUDY OF COLOMBIAN YLANG-YLANG (CANANGA-ODORATA) OILS OBTAINED BY DIFFERENT EXTRACTION TECHNIQUES/

Steam distillation (SD), simultaneous distillation-solvent extraction (SDE), and supercritical (CO2) extraction (SFE) were used to isolate v olatile secondary metabolites from fresh, totally mature flowers of Co lombian ylang-ylang (Cananga odorata). The various extracts were analy zed by capillary chromatography (DB-1, DBWAX, 60 m columns) using FID, NPD or MSD (EI, 70 eV). Kovats indexes, mass spectra, or standard sub stances were employed for compound identification. 51, 70, and 73 comp ounds at concentrations above 100 ppb were detected in the SD, SDE, an d SFE extracts, respectively. The main constituents of these extracts mere linalool (20.7, 28.0, and 16.5%), germacrene-D (10.1, 3.1, and 20 .3%) benzyl benzoate (14.1, 2.9, and 3.9%), benzyl acetate (9.6, 17.0, and 6.2%), caryophyllene (3.1, 2.9, and 3.9%), and p-methylanisole (6 .8, 6.1, and 2.7%). 85% of the composition of SDE extracts was represe nted by oxygenated compounds. Heavy hydrocarbons (C-n >20) and fatty a cids were found only in the SFE extracts, which also had a higher cont ent of nitrogenated compounds (phenylacetonitrile, 4-methylbenzaldoxim e, indole, 2-phenyl-nitroethane, and methyl anthranilate) and sesquite rpenes (43% vs 19.5% in SD and 8.1% in SDE) and 1.5-2 times lower conc entration of monoterpenes and light oxygenated compounds than the SD ( 49.7%) and SDE (64.5%) extracts.