CLONING AND EXPRESSION OF A BETA-1,4-ENDOGLUCANASE GENE FROM CELLULOMONAS SP CE1B7 IN ESCHERICHIA-COLI, PURIFICATION CHARACTERIZATION OF THE RECOMBINANT ENZYME

Authors
FULOP L TRAN SLP PRAGAI Z FELFOLDI F PONYI T
Citation
L. Fulop et al., CLONING AND EXPRESSION OF A BETA-1,4-ENDOGLUCANASE GENE FROM CELLULOMONAS SP CE1B7 IN ESCHERICHIA-COLI, PURIFICATION CHARACTERIZATION OF THE RECOMBINANT ENZYME, FEMS microbiology letters, 145(3), 1996, pp. 355-360
Citations number
21
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
145
Issue
3
Year of publication
1996
Pages
355 - 360
Database
ISI
SICI code
0378-1097(1996)145:3<355:CAEOAB>2.0.ZU;2-3
Abstract
A gene library of a newly isolated Cellulomonas sp. strain was constru cted in Escherichia coli and clones were screened for endoglucanase ac tivity using dye-labelled carboxymethylcellulose. Seventeen clones wer e isolated that carried DNA inserts coding for endoglucanase enzymes. Of the 17 clones, one carrying the gene cegA, was further characterize d. The recombinant endoglucanase was purified by FPLC. The endoglucana se was active against carboxymethylcellulose, lichenin and also degrad ed crystalline cellulose and birchwood xylan. The molecular mass of th e enzyme (36 kDa), and its pH (7.4) and temperature (35 degrees C) opt ima were determined.