DEVELOPMENT OF A NOVEL CONDUCTOMETRIC DETERMINATION OF ORGANOPHOSPHATE INSECTICIDES USING PHOSPHOTRIESTERASE

Authors
SODE K TOGO H YAMAZAKI T TSUGAWA W OHUCHI S NARITA M YAGIUDA K
Citation
K. Sode et al., DEVELOPMENT OF A NOVEL CONDUCTOMETRIC DETERMINATION OF ORGANOPHOSPHATE INSECTICIDES USING PHOSPHOTRIESTERASE, Denki Kagaku Oyobi Kogyo Butsuri Kagaku, 64(12), 1996, pp. 1234-1238
Citations number
17
Categorie Soggetti
Electrochemistry
Journal title
Denki Kagaku Oyobi Kogyo Butsuri Kagaku → ACNP
ISSN journal
03669297
Volume
64
Issue
12
Year of publication
1996
Pages
1234 - 1238
Database
ISI
SICI code
0366-9297(1996)64:12<1234:DOANCD>2.0.ZU;2-K
Abstract
Phosphotriesterase (PTE) from a bacterium, Flavobacterium sp. was appl ied for a novel biochemical determination of organophosphorous insecti cides, in conjunction with a conductometry. Paraoxon or fenitrothion w as hydrolyzed by PTE causing conductance change, which was subsequentl y determined by a conductance electrode. Under optimum condition, para oxon and fenitrothion were determined within the range of 1.0 similar to 7.0 mu M and 20-100 mu M, respectively.