CHARACTERIZATION OF TRICHURIS-SKRJABINI BY ISOENZYME GEL-ELECTROPHORESIS - COMPARATIVE-STUDY WITH TRICHURIS-OVIS

Morphological and biometric studies were performed in Trichuris skrjab ini (Baskakov, 1924) collected from the caecum of Capra hircus. The LD H (EC 1.1.1.27.), G6PD (EC 1.1.1.49.), GPI (EC 5.3.1.9.), MDH (EC 1.1. 1.37) and malic enzyme (ME) (EC 1.1.1.40) isoenzymatic patterns of T. skrjabini were determined by starch gel electrophoresis. The G6PD and GPI isoenzymatic patterns of T. skrjabini displayed two anodic bands f or both enzymes: one fast migration band and one band near the origin. This isoenzymatic pattern was interpreted as two gene loci encoding b oth enzymes. The LDH isoenzymatic pattern of T. skrjabini was characte rized by the presence of a cathodically migrating band, while the MDH isoenzymatic pattern showed a very slow cathodic band. These two pheno types were interpreted as the expression of a homozygous state of a ge ne locus for LDH and MDH in T. skrajabini. The ME isoenzymatic pattern was characterized by the presence of a single anodic band. Further, c omparative isoenzymatic studies were carried out between T. skrajabini and T. ovis. The different G6PD, GPI, LDH, MDH and ME isoenzymatic pa tterns observed for both species allowed us to distinguish them and th erefore to use isoenzymatic patterns as a diagnostic tool to different iate species of Trichuris.