SYNTHESIS AND ACTIVITIES OF A THIENYL DIHYDROPYRIDINE SERIES ON INTRACELLULAR CALCIUM IN A RAT PITUITARY CELL-LINE (GH3 B6)/

The synthesis of a thienyl dihydropyridine series according to the Han tzsch method is described. The influence of these derivatives on intra cellular calcium ([Ca2+]i) in GH3 cells was evaluated in vitro using s pectrofluorimetry with indol as Ca2+ fluorescent probe. We compared th eir effects on [Ca2+]i and hormone release with those of nifedipine. T he most active tested compounds on [Ca2+]i were those methylated on th e 3-position of the thienyl ring (activity was about 75% of nifedipine ). Interestingly, the most efficient compounds on [Ca2+]i were also th e most efficient on hormone release.