A. Matsumori et al., INOTROPIC AGENTS DIFFERENTIALLY INHIBIT THE INDUCTION OF NITRIC-OXIDESYNTHASE BY ENDOTOXIN IN CULTURED MACROPHAGES, Life sciences, 59(10), 1996, pp. 121-125
Citations number
18
Categorie Soggetti
Biology,"Medicine, Research & Experimental","Pharmacology & Pharmacy
We investigated the effects of inotropic agents with phosphodiesterase
III inhibitory properties, amrinone, pimobendan and vesnarinone, and
cell permeable cyclic nucleotide analogue, 8-bromo adenosine 3'5'-cycl
ic monophosphate (8 Br-cAMP) on the induction of nitric oxide synthase
(NOS) by lipopolysaccharide in J774A.1 macrophages in vitro. Although
all three inotropic agents inhibited nitrite accumulation, the degree
of inhibition was different, with pimobendan being the most potent in
hibitor and amrinone the least. Vesnarinone inhibited nitrite formatio
n biphasically. 8 Br-cAMP increased nitrite production at high concent
rations, suggesting that the inhibitory effects of inotropic agents co
uld not be explained by an increase in cAMP. Although differential inh
ibition of inducible NOS by inotropic agents may explain the different
effects of these drugs in patients with heart failure, further study
is necessary to reach this conclusion.