DENATURING GRADIENT GEL-ELECTROPHORESIS - A NOVEL METHOD FOR DETERMINING RH PHENOTYPE FROM GENOMIC DNA

Denaturing gradient gel electrophoresis (DGGE) was carried out on PCR products amplified from exons 2 and 5 of RHD and RHCE. Exon 2 of RHD a nd exon 2 of the C allele of RHCE have an identical sequence, which di ffers from that of the c allele of RHCE. One band representing D and/o r C, and another representing c, could be distinguished by DGGE of exo n 2 amplifications of genomic DNA from individuals with the appropriat e Rh phenotype. C and c could only be distinguished in D-negative samp les. Exon 5 of RHD and exon 5 of the E and e alleles of RHCE all have different nucleotide sequences. Bands representing D, E and e could be distinguished following DGGE of the products of exon 5 amplification of genomic DNA from individuals with red cells of the appropriate Rh p henotype. In samples from individuals with VS+ red cells (V+ or V-) th ere was a shift of the band representing e. Sequencing demonstrated th at VS is associated with a RHCE e sequence with a single base change p redicting a Leu245 --> Val substitution in the Rh polypeptide. This su bstitution may be responsible for the VS and e(s) antigens.