F. Qing et al., IN-VIVO QUANTIFICATION OF HUMAN PULMONARY BETA-ADRENOCEPTOR DENSITY USING PET - COMPARISON WITH IN-VITRO RADIOLIGAND BINDING, The Journal of nuclear medicine, 37(8), 1996, pp. 1275-1281
A new method has recently been developed to quantify pulmonary beta-ad
renergic receptors in vivo using PET. This study used in vitro radioli
gand binding assay (PLEA) as the gold standard to validate in vivo PET
measurements. Methods: Five male patients with lung cancer aged 57 yr
(range 42-67 yr) were studied. PET scanning was performed the day bef
ore thoracotomy to determine regional pulmonary beta-receptor density.
RLBA was carried out on cell membranes prepared from specimens of lun
g tissue obtained at the thoracotomy to measure beta-receptor density
in vitro. In both cases, the hydrophilic nonselective beta-antagonist
radioligand (S)-CGP-12177 was used. For PET studies, this was labeled
with C-11 and for RLBA with H-3. Results: In the PET study, beta-recep
tor density (B-max) was 9.43 +/- 1.32 pmole g(-1) tissue. In the RLBA
study, B-max was 99.0 +/- 15.5 fmole mg(-1) protein, equivalent to 9.9
0 +/- 1.55 pmole g(-1) tissue. These values are in good agreement with
previously reported in vitro measurements on human lung membranes usi
ng I-125-iodocyanopindolol. A correlation was found between beta-adren
ergic density obtained using PET and beta-adrenergic density obtained
using RLBA (r = 0.92; p < 0.05). Conclusion: The results support the u
se of PET as a new method for imaging and quantifying pulmonary beta-a
drenergic receptors in vivo, opening the way for studies of physiologi
cal and pharmacological regulation of beta-adrenergic receptors throug
h noninvasive serial measurements.