ASSESSMENT OF A STANDARDIZED REVERSE-TRANSCRIPTASE PCR ASSAY FOR QUANTIFYING HIV-1 RNA IN PLASMA AND SERUM

Citation
J. Izopet et al., ASSESSMENT OF A STANDARDIZED REVERSE-TRANSCRIPTASE PCR ASSAY FOR QUANTIFYING HIV-1 RNA IN PLASMA AND SERUM, Journal of virological methods, 60(2), 1996, pp. 119-129
Citations number
30
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
ISSN journal
01660934
Volume
60
Issue
2
Year of publication
1996
Pages
119 - 129
Database
ISI
SICI code
0166-0934(1996)60:2<119:AOASRP>2.0.ZU;2-K
Abstract
The analytical variability of the new commercially available Reverse T ranscriptase-Polymerase Chain Reaction (RT-PCR) assay, Amplicor(TM) HI V-I Monitor(TM), has been assessed to establish criteria for assessing the significance of HIV-I RNA level measurements. Estimations of the standard deviations (SD) of log-copies in inter-assay (mean 0.09 log) and in inter-laboratory (mean 0.14 log) reproducibility experiments de monstrated that the assay can discriminate with 95% confidence between 3-fold (inter-assay) and 5-fold differences (inter-laboratory). The i nter-lot reproducibility (mean 0.10 log) was similar to the inter-assa y reproducibility. The HIV-I RNA concentrations measured in plasma col lected in potassium EDTA anticoagulant were slightly higher than those measured in plasma collected in sodium citrate. The HIV-1 RNA concent rations measured in sera were about 50%, of the HIV-1 RNA concentratio ns measured in paired plasma samples. However, there was a strong corr elation between these two measurements (P<0.0001). The assay was used to measure viral RNA in the plasma of 50 HIV-1 positive individuals at different stages of infection. All the individuals had detectable HIV -I RNA (300-957 000 copies/ml). There was no correlation between HIV-I RNA and Immune Complex Dissociated (ICD) p24 antigen, but HIV-I RNA w as correlated with CD4+cell counts (P<0.0001) and the clinical stage ( P=0.0042), with higher HIV-1 RNA concentrations in patients with a mor e advanced stage of the disease. The significant association of HIV-1 RNA with major markers of HIV infection and the reliability of this se nsitive, easy-to-use RT-PCR assay indicate its suitability for use in clinical trials and suggest that this assay is appropriate for routine clinical applications.