STABILITY OF INVERTASE IN REVERSE MICELLES

Authors
SUBRAMANI S SHAH C MADAMWAR D
Citation
S. Subramani et al., STABILITY OF INVERTASE IN REVERSE MICELLES, Applied biochemistry and biotechnology, 60(1), 1996, pp. 33-39
Citations number
17
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Applied biochemistry and biotechnology → ACNP
ISSN journal
02732289
Volume
60
Issue
1
Year of publication
1996
Pages
33 - 39
Database
ISI
SICI code
0273-2289(1996)60:1<33:SOIIRM>2.0.ZU;2-X
Abstract
The stability of invertase was studied under various conditions, inclu ding at 75 degrees C, in presence of stabilizers (sorbitol and glycero l) at 75 degrees C, and in the presence of denaturants (urea and trich loroacetic acid) at 37 degrees C in reverse micelles. Stability of the invertase in reverse micelles was found to be improved over that of t he enzyme in bulk aqueous solution. Sorbitol could enhance enzyme stab ility as it does in the bulk aqueous system. The stabilizing effect of glycerol was reduced in reverse micelles. The denaturation pattern of urea remains unaltered. However, the denaturation effect of trichloro acetic acid has been reduced in reverse micelles.