KINETICS OF CHOLESTEROL OXIDATION BY CHOLESTEROL OXIDASE

Authors
VASUDEVAN PT ZHOU T
Citation
Pt. Vasudevan et T. Zhou, KINETICS OF CHOLESTEROL OXIDATION BY CHOLESTEROL OXIDASE, Applied biochemistry and biotechnology, 60(1), 1996, pp. 63-72
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Applied biochemistry and biotechnology → ACNP
ISSN journal
02732289
Volume
60
Issue
1
Year of publication
1996
Pages
63 - 72
Database
ISI
SICI code
0273-2289(1996)60:1<63:KOCOBC>2.0.ZU;2-T
Abstract
Kinetic studies of cholesterol oxidation catalyzed by soluble choleste rol oxidase from Brevibacterium were conducted. The optimum temperatur e and pH were found to be 40-45 degrees C and 7.0, respectively. A plo t of initial reaction rate versus cholesterol concentration is sigmoid al in shape. Analysis of the data suggests that the reaction follows a concerted model and not a stepwise model.