DIFFERENTIAL STAINING OF HUMAN ERYTHROCYTE-MEMBRANE PROTEINS BY COOMASSIE AND SILVER - IMPLICATIONS IN THE ASSESSMENT OF HOMOGENEITY OF MEMBRANE-PROTEINS

Authors
PIKULA S BANDOROWICZPIKULA J AWASTHI S AWASTHI YC
Citation
S. Pikula et al., DIFFERENTIAL STAINING OF HUMAN ERYTHROCYTE-MEMBRANE PROTEINS BY COOMASSIE AND SILVER - IMPLICATIONS IN THE ASSESSMENT OF HOMOGENEITY OF MEMBRANE-PROTEINS, Biochemical archives, 12(4), 1996, pp. 237-243
Citations number
30
Categorie Soggetti
Biology
Journal title
Biochemical archives → ACNP
ISSN journal
07495331
Volume
12
Issue
4
Year of publication
1996
Pages
237 - 243
Database
ISI
SICI code
0749-5331(1996)12:4<237:DSOHEP>2.0.ZU;2-C
Abstract
Non-uniform staining of electrophoretically separated polypeptides is one of the major problems in the assessment of their homogeneity. In t he present communication we report the example of such behavior of hum an erythrocyte membrane and cytoskeleton proteins, stained with Coomas sie brilliant blue R-250, Silver nitrate, and Periodic acid-Schiff's ( PAS) in reduced denaturing gels.