A COMPARISON OF FAST-ATOM-BOMBARDMENT AND ELECTROSPRAY AS METHODS OF IONIZATION IN THE STUDY OF SULFATED-LIPIDS AND SULFONATED-LIPIDS BY TANDEM MASS-SPECTROMETRY

Authors
GRIFFITHS WJ BROWN A REIMENDAL R YANG Y ZHANG J SJOVALL J
Citation
Wj. Griffiths et al., A COMPARISON OF FAST-ATOM-BOMBARDMENT AND ELECTROSPRAY AS METHODS OF IONIZATION IN THE STUDY OF SULFATED-LIPIDS AND SULFONATED-LIPIDS BY TANDEM MASS-SPECTROMETRY, Rapid communications in mass spectrometry, 10(10), 1996, pp. 1169-1174
Citations number
31
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
Journal title
Rapid communications in mass spectrometry → ACNP
ISSN journal
09514198
Volume
10
Issue
10
Year of publication
1996
Pages
1169 - 1174
Database
ISI
SICI code
0951-4198(1996)10:10<1169:ACOFAE>2.0.ZU;2-J
Abstract
In recent years electrospray has become the most popular ionization te chnique for the analysis of biomolecules. In the present study, we hav e compared the electrospray and continuous-how fast-atom bombardment m ethods of sample introduction and ionization for the analysis of sulph ated and sulphonated lipids by tandem mass spectroscopy. The tandem ma ss spectra obtained when using the two ionization techniques were simi lar; however, small differences were evident. These differences indica te that the precursor ions generated by fast-atom bombardment and elec trospray have different internal energies. The two ionization techniqu es give similar sensitivities for the recording of collision-induced d issociation spectra. Complete structural information can be obtained f rom 5 to 50 ng of lipid.