SIMPLE ANALYSIS OF PLASMALOGENS IN ERYTHROCYTES USING GAS-CHROMATOGRAPHY MASS-SPECTROMETRY WITH SELECTED-ION MONITORING ACQUISITION

Plasmalogens are a unique class of ether-phospholipids whose role, eve n though not fully defined, is essential. Their biosynthesis starts in peroxisomes, therefore, plasmalogen analysis is fundamental in the st udy of peroxisomal disorders. The present work reports a simple method for plasmalogen determination in erythrocytes for use in the study of peroxisomal disorders in humans; the procedure is based on two other methods that have previously been reported and employs GC/MS for separ ation and detection.