TRANSCRIPTION, TRANSLATION, AND CELLULAR-LOCALIZATION OF 3 AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS STRUCTURAL PROTEINS - ODV-E18,ODV-E35, AND ODV-EC27

This paper identifies two structural proteins of the occluded derived viral envelope of Autographa californica nuclear polyhedrosis virus (A cMNPV): ODV-E18 and ODV-E35. In addition, we identify a protein, ODV-E C27, that Is incorporated into the capsid of occluded virus, which is not detected in budded virus. The genes for these proteins reside with in the IE0 intron. The intron was sequenced, and five open reading fra mes (ORF) were identified. ORF 3 (genomic ORF 143) codes for the ODV e nvelope protein, ODV-E18. ORF 4 (genomic ORF 144) codes for ODV-EC27, and Western blot analyses locate this protein to both the ODV capsid a nd envelope. Transcripts for both ODV-E18 and ODV-EC27 initiate from c onserved TAAG motifs, and transcripts are detected from 16 through 72 hr p.i, Antiserum to ODV-E18 recognizes a band of 18 kDa on Western bl ots of extracts from infected cells and bands of 18 and 35 kDa on West ern blots of proteins from purified ODV envelope. N-terminal amino aci d sequencing reveals that both ODV-E18 and ODV-E35 contain the same N- terminus. Antiserum to ODV-EC27 recognizes a protein of 27 kDa on West ern blots of extracts from infected cells and bands of 27 and 35 kDa o n Western blots of proteins from purified ODV. Using immunogold labeli ng techniques, ODV-E18 and/or ODV-E35 are detected in viral induced in tranuclear microvesicles and are not detected in the plasma membrane, cytoplasmic membranes, or the nuclear envelope. Immunogold labeling us ing antisera to ODV-EC27 detects this protein on both the ODV envelope and capsid. (C) Academic Press, Inc.