EFFECT OF TEMPERATURE AND VISCOSITY OF SIEVING MEDIUM ON ELECTROPHORETIC BEHAVIOR OF SODIUM DODECYL SULFATE-PROTEINS ON CAPILLARY ELECTROPHORESIS IN PRESENCE OF PULLULAN

Pullulan was used as a sieving medium in high-performance capillary ge l electrophoresis to study the effect of temperature and viscosity on electrophoretic behavior of sodium dodecyl sulfate (SDS)-proteins with molecular masses in the range of 14 400-116 000 Da. The migration tim e decreased with increasing capillary temperature. Equations were deri ved relating mobility of SDS-proteins to capillary temperature and vis cosity of pullulan solution. Linear relationships were obtained from e xperiments between logarithm of mobility and reciprocal temperature an d between double logarithmic values of mobility and viscosity of pullu lan solution. The experimental results agree well with the equations.