R. Kannan et al., TRANSFORMING GROWTH-FACTOR-ALPHA OVEREXPRESSION IN PROLIFERATIVE VERRUCOUS LEUKOPLAKIA AND ORAL SQUAMOUS-CELL CARCINOMA - AN IMMUNOHISTOCHEMICAL STUDY, Oral surgery, oral medicine, oral pathology, oral radiology and endodontics, 82(1), 1996, pp. 69-74
Citations number
42
Categorie Soggetti
Pathology,Surgery,"Dentistry,Oral Surgery & Medicine
Proliferative verrucous leukoplakia is a unique type of oral leukoplak
ia that has a high risk of malignant transformation. The aim of this s
tudy was to examine the expression of transforming growth factor-alpha
in proliferative verrucous leukoplakia, oral squamous cell carcinoma,
and normal mucosa. Transforming growth factor-alpha, a potent mitogen
, is known to play an important role in various neoplasms including or
al squamous cell carcinoma. Immunohistochemical localization of transf
orming growth factor-alpha in archival paraffin-embedded sections was
performed with commercially available monoclonal antibodies. Ten cases
each ai normal mucosa, proliferative verrucous leukoplakia, and oral
squamous cell carcinoma were stained. Quantification oi the staining i
ntensity, expressed as the cytoplasmic optical density, was done with
the Roche Image Analysis System. The data were statistically analyzed
with the one-way analysis of variance and Tukey tests. Notably, the me
an cytoplasmic optical density of proliferative verrucous leukoplakia
was significantly higher than the mean cytoplasmic optical density of
normal mucosa (p < 0.01). squamous cell carcinoma, however, this diffe
rence was not significant (p > 0.05). The mean cytoplasmic optical den
sity values demonstrate that increased transforming growth factor-alph
a immunoreactivity occurs in proliferative verrucous leukoplakia and o
ral squamous cell carcinoma relative to normal mucosa.