H. Onaga et al., PROTECTIVE IMMUNITY INDUCED WITH THE ANTIGENS FRACTIONATED BY AFFINITY-CHROMATOGRAPHY FROM THE 2ND-GENERATION SCHIZONTS OF LEUCOCYTOZOON-CAULLERYI, Journal of veterinary medical science, 58(7), 1996, pp. 677-679
A crude extract of the 2nd generation schizonts (2GS) of Leucocytozoon
caulleryi and chicken serum containing serum-soluble antigens were ap
plied to an immunoaffinity column using immunoglobulin of chickens rec
overed from L. caulleryi infections. The fractionated antibody-bound p
roteins showed several bands between 70 kDa and 35 kDa by the electrop
horetic analysis. By immunization of chickens, the fractionated protei
ns conferred the protection at varying degrees comparable to that conf
erred by the unfractionated original materials. These results indicate
d that antiserum against 2GS can recognize the proteins involved in pr
otective immunity.