PROTECTIVE IMMUNITY INDUCED WITH THE ANTIGENS FRACTIONATED BY AFFINITY-CHROMATOGRAPHY FROM THE 2ND-GENERATION SCHIZONTS OF LEUCOCYTOZOON-CAULLERYI

A crude extract of the 2nd generation schizonts (2GS) of Leucocytozoon caulleryi and chicken serum containing serum-soluble antigens were ap plied to an immunoaffinity column using immunoglobulin of chickens rec overed from L. caulleryi infections. The fractionated antibody-bound p roteins showed several bands between 70 kDa and 35 kDa by the electrop horetic analysis. By immunization of chickens, the fractionated protei ns conferred the protection at varying degrees comparable to that conf erred by the unfractionated original materials. These results indicate d that antiserum against 2GS can recognize the proteins involved in pr otective immunity.