12-WELL CULTURE PLATE FOR THE EFFICIENT COLLECTION AND CULTURE OF HUMAN OOCYTES AND EMBRYOS

Objective: Our goal was to assess a 12-well oocyte collection and embr yo culture plate for use in the NF laboratory. Design: This was a pros pective nonrandomized study. Setting: The setting was a university in vitro fertilization program. Patients: Eighty-four consecutive inferti lity couples presenting for IVF were studied. Main Outcome Measures: T he main outcome measure was pregnancy (delivered). Results: A 34% deli vered pregnancy rate per retrieval was attained using the 12-well coll ection and culture plate without the use of expensive culture media an d special serum supplementation. Conclusions: The use of a 12-well pla te for oocyte collection and embryo culture provides a simple, economi cal, efficient, and effective means of producing human embryos during in vitro fertilization. This system is capable of supporting high rate s of ongoing and delivered pregnancies.