SYNTHESIS AND ANTICOAGULANT ACTIVITY OF SULFATED GLUCOSIDE-BEARING POLYMER

Poly(glucosyloxyethyl methacrylate) sulfate [poly(GEMA) sulfate], whic h contains sulfated glucoside residues, was prepared by the reaction w ith N,N-dimethylformamide (DMF)/sulfur trioxide (SO3) complex. The deg ree of sulfation was easily controlled by changing the amount of DMF/S O3 complex added and reaction time. The total human blood clotting tim e in the presence of poly(GEMA) sulfate was prolonged by increasing th e dose or the degree of sulfation of the polymer. The anticoagulant ac tivity of poly(GEMA) sulfate was also compared with that of dextran su lfate, poly(styrenesulfonic acid), poly(vinylsulfuric acid), and hepar in. The result suggests that sulfated saccharide residues are essentia l for endowing anticoagulant activity to synthetic polymer.