Citation
M. Macor et al., CLONING AND TRANSFER OF SUCRASE OPERON IN ESCHERICHIA-COLI, Journal of microbiological methods, 26(1-2), 1996, pp. 119-124
Citations number
23
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
ISSN journal
01677012
Volume
26
Issue
1-2
Year of publication
1996
Pages
119 - 124
Database
ISI
SICI code
0167-7012(1996)26:1-2<119:CATOSO>2.0.ZU;2-J
Abstract
The sucrase operon was transferred from the donor Escherichia coli MM2
3 strain by the phage P1 into the Escherichia coli TG1, mini-Mu phage
into the strain Escherichia coli JT4 and subsequently to the basic clo
ning vectors. We have prepared strains of Escherichia coli XL1 Blue, E
scherichia coli JM109 that are able to grow on various concentrations
of sucrose. We also transferred the sucrase operon by conjugation of m
obile plasmid pUR400 into the strain Escherichia coli MC4100.