Many genes in plant mitochondria have been analyzed in the past 15 yea
rs and regulatory processes controlling gene expression can now be inv
estigated. In vitro systems capable of initiating transcription faithf
ully at promoter sites have been developed for both monocot and dicot
plants and will allow the identification of the interacting nucleic ac
id elements and proteins which specify and guide transcriptional activ
ities. Mitochondrial activity, although required in all plant tissues,
is capable of adapting to specific requirements by regulated gene exp
ression. Investigation of the factors governing the quality and quanti
ty of distinct RNAs will define the extent of interorganelle regulator
y interference in mitochondrial gene expression.