K. Wrobelzasada et al., APPLICATION OF INTERNAL STANDARD FOR DERIVATIVE-SPECTROPHOTOMETRIC DETERMINATION OF AZINPHOS-METHYL IN COMMERCIAL FORMULATIONS, Talanta, 43(7), 1996, pp. 1055-1060
The use of an internal standard is proposed in this work for first der
ivative spectrophotometric determination of azinphos in formulations.
Generally, the spectrophotometric procedure is simpler and less expens
ive than chromatographic techniques recommended for the analysis of pe
sticides. However, while determining the pesticide in commercial formu
lations the, many-fold dilution required for such sensitive detection
is a serious source of analytical error. It is known that an internal
standard (IS), if properly chosen, can help eliminate this type of pro
blem. A mixture of acetophenone (used as an IS in the high performance
liquid chromatography (HPLC) procedure) and the blue dye Erioglaucine
(A) was applied for the determination of azinphos-methyl in commercia
l formulations. To ensure the best conditions for the zero-crossing te
chnique, the composition of the mixture was optimized to obtain the ze
ro value of the first derivative absorbance of the IS at a minimum of
the azinphos-methyl first derivative absorbance. Also, at the maximum
of the first derivative spectrum of the IS, the differential absorptio
n signal of the analyte was negligible. Analytical characteristics for
the first derivative spectrophotometric procedure proposed were evalu
ated (r(2) = 0.9998, detection limit = 0.043 mg, quantification limit
= 0.143 mg) and the analytical. results obtained (35.02 +/- 0.20% of a
zinphos-methyl in the formulation) were in good agreement with the res
ults obtained using the official HPLC method (35.44 +/- 0.32%).