PERMSELECTIVE-MEMBRANE PYROLYTIC-GRAPHITE ELECTRODE FOR THE STUDY OF MICROVOLUMES OF [2FE-2S] FERREDOXIN

Authors
HALADJIAN J THIERRYCHEF I BIANCO P
Citation
J. Haladjian et al., PERMSELECTIVE-MEMBRANE PYROLYTIC-GRAPHITE ELECTRODE FOR THE STUDY OF MICROVOLUMES OF [2FE-2S] FERREDOXIN, Talanta, 43(7), 1996, pp. 1125-1130
Citations number
19
Journal title
Talanta
ISSN journal
00399140 → ACNP
Volume
43
Issue
7
Year of publication
1996
Pages
1125 - 1130
Database
ISI
SICI code
0039-9140(1996)43:7<1125:PPEFTS>2.0.ZU;2-X
Abstract
A permselective-membrane pyrolytic graphite electrode was used to stud y the electrochemistry of [2Fe-2S] ferredoxin entrapped between the me mbrane and the graphite surface, in the presence of poly(L-lysine). Fa ctors influencing the electrode response [such as the concentration of poly(L-lysine), ionic strength and pH] were explored. The analytical performance of this permselective-membrane electrode, which allows 20 pmol of ferredoxin to be detected, was examined.