ADAPTATION OF MICROTHERMAL PROBES FOR THE DETERMINATION OF BIOCHEMICAL SPECIES

A glucose sensitive enzymatic thermal sensor based on a pair of commer cial microthermal probes was fabricated by cross-linking of the corres ponding enzymes (glucose oxidase and catalase) with bovine serum album in onto the chip surfaces. The characteristics of this sensor were opt imised by testing it in different conditions. To find the maximal sens itivity, the calibration curves of this sensor were measured in differ ent buffer solutions with and without the addition of H2O2 to the anal ysed samples at various rates of sample flow stream. Different buffer solutions and hydrogen peroxide concentraions were used to realise the principles of a chemical and biochemical amplification of the biosens or response. It was shown that the level of the biosensor response and its dynamic range increase if hydrogen peroxide is added to the analy sed samples and depend on the type of buffer solutions tested and the rate of the sample flow stream.