DOPAMINERGIC CONTROL OF LIGHT-ADAPTIVE SYNAPTIC PLASTICITY AND ROLE IN GOLDFISH VISUAL BEHAVIOR

Dopamine has been implicated in processes of retinal light and dark ad aptation, In goldfish retina, horizontal cell dendrites elaborate neur ite processes (spinules) into cone terminals, in a light- and dopamine -dependent manner. However, the functions of retinal dopamine and the horizontal cell spinules in visual behavior are unknown. These issues were addressed in behavioral, electroretinographic, and anatomical stu dies of normal fish and those with unilateral depletion of retinal dop amine induced by intraocular (i.o.) injections with 6-hydroxydopamine (6-OHDA). Dopamine interplexiform cells (DA-IPC) disappear within 2 we eks after 6-OHDA injection; cell bodies appear at the marginal zone wi thin 6 weeks at which time neurites slowly reinnervate the retina with a sparse plexus over the next 12 months. We found that dopamine deple tion increased light sensitivity at photopic but not scotopic backgrou nds by 2.5 log units, an effect mimicked by i.o. injections of dopamin e D1 and D2 antagonists, The ERG b-wave increment thresholds were the same for control and dopamine depleted eyes, indicating a normal trans ition from rod to cone systems in the ON pathway, Light-dependent spin ule formation was reduced by about 60% in dopamine-depleted retinas, b ut returned to normal by 3 months and 9 months after injection in the entire retina, even areas not directly innervated with DA-IPC processe s. Spinule formation in vivo was inhibited 50% with i.o. injection of SCH 23390 in control retinas as well as throughout 3 month 6-OHDA inje cted retinas, including DA-IPC free areas, This latter result indicate s a volume effect of dopamine, diffusing laterally through the retina over several millimeters, in regulating spinules. We conclude that DA- IPCs regulate sensitivity to background at photopic levels not via the ON pathway, but perhaps the OFF pathway, Goldfish display both increa sed sensitivity to light and a normal Purkinje shift in the ERG b-wave whether or not horizontal cell spinules are present, indicating that dopamine control of photopic vision in fish is not mediated through li ght-induced spinule formation of horizontal cell dendrites. Copyright (C) 1996 Elsevier Scence Ltd.