AMINO-ACID-SEQUENCE OF CATECHOL 1,2-DIOXYGENASE (PYROCATECHASE) ISOZYME ALPHA-ALPHA FROM PSEUDOMONAS-ARVILLA C-1

Authors
NAKAI C YAMAZAKI G KAGAMIYAMA H NOZAKI M
Citation
C. Nakai et al., AMINO-ACID-SEQUENCE OF CATECHOL 1,2-DIOXYGENASE (PYROCATECHASE) ISOZYME ALPHA-ALPHA FROM PSEUDOMONAS-ARVILLA C-1, Biochemistry and molecular biology international, 39(4), 1996, pp. 781-788
Citations number
27
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
39
Issue
4
Year of publication
1996
Pages
781 - 788
Database
ISI
SICI code
1039-9712(1996)39:4<781:AOC1(I>2.0.ZU;2-W
Abstract
Catechol 1,2-dioxygenase catalyzes the oxygenative ring cleavage of ca techol to form cis,cis-muconic acid, and contains a ferric form of iro n as its sole cofactor. Pseudomonas arvilla C-1 contains three isozyme s of the enzyme, alpha alpha, alpha beta, and beta beta [C. Nakai et a l. (1990) J. Biol. Chem. 265, 660-665]. We have determined the amino a cid sequence of the alpha alpha isozyme by direct analysis. The sequen ce shared 77% homology with isozyme beta beta, and had conserved tyros yl and histidyl residues which are thought to be involved in the bindi ng of ferric ion.