OPPOSITE EFFECTS OF BASIC FIBROBLAST GROWTH-FACTOR AND HEPARIN ON HEPARIN HEPARAN SULFATE DEGRADING ENZYMES FROM FLAVOBACTERIUM-HEPARINUM/

Authors
CAVARI S VANNUCCHI S
Citation
S. Cavari et S. Vannucchi, OPPOSITE EFFECTS OF BASIC FIBROBLAST GROWTH-FACTOR AND HEPARIN ON HEPARIN HEPARAN SULFATE DEGRADING ENZYMES FROM FLAVOBACTERIUM-HEPARINUM/, Biochemistry and molecular biology international, 39(4), 1996, pp. 823-831
Citations number
22
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
39
Issue
4
Year of publication
1996
Pages
823 - 831
Database
ISI
SICI code
1039-9712(1996)39:4<823:OEOBFG>2.0.ZU;2-B
Abstract
Heparinase I is normally inhibited by an excess or substrate; in this paper we describe that bFGF counteracts the inhibiting effect of the s ubstrate and that heparinase I activity is increased in the presence o f bFGF. This effect was observed using heparin concentrations ranging from 10(-9) to 10(-7) M with either a ten fold molar excess or an equi molar concentration of bFGF. In addition, the degree of depolymerizati on of heparan sulfate produced by heparitinase from Flavobacterium hep arinum was increased in the presence of bFGF.