TIN-PROTOPORPHYRIN PLUS PHOTOIRRADIATION INDUCES A RELEASE OF ARYLSULFATASE FROM LYSOSOMES OF RAT HEPATOCYTES IN-VITRO AND IN-VIVO

The effects of the combination of treatments with tin-protoporphyrin ( SnPP) and irradiation on hepatic lysosomes were examined in vitro and in vivo. SnPP caused no release of arylsulfatase from lysosomes in the dark, but the combined treatment with irradiation induced the release of arylsulfatase from lysosomes in vitro. The positive correlation be tween incident photoenergy and the release of arylsulfatase was analyz ed. The administration of L-ascorbic acid prevented the release of the enzyme in a dose-dependent manner. The irradiation-induced release of arylsulfatase from lysosomes occurred in hepatocytes, which are known to concentrate SnPP. Studies using histochemical and biochemical meth ods revealed the release of arylsulfatase from lysosomes in the hepato cytes of SnPP-injected rats.