Em. Hol et al., DIFFERENTIAL-EFFECTS OF ACTH-RELATED PEPTIDES AND FORSKOLIN ON RAT SCHWANN-CELL PROLIFERATION AND LOW-AFFINITY NGF RECEPTOR EXPRESSION, Neuroscience research communications, 19(1), 1996, pp. 37-45
We investigated the effect of the neurotrophic peptides alpha MSH and
Org 2766 on Schwann cell proliferation (BrdU incorporation) and low af
finity nerve growth factor receptor (LNGFR) expression in vitro. Cells
were cultured under four conditions: low or high density culture, wit
h forskolin present or absent in the medium during one week before sta
rt of the experiment. Schwann cells were treated with alpha MSH, Org 2
766 or forskolin. After 48h forskolin (10 mu M) increased BrdU incorpo
ration (32-151%) in all culture conditions and LNGFR production (25-19
4%) in 3 out of 4 conditions. alpha MSH and Org 2766 had no effect. Th
is study shows that Schwann cell proliferation and LNGFR expression is
under control of cAMP. Furthermore, the neurotrophic effect of alpha
MSH and Org 2766 is probably not mediated through an increased cell di
vision or LNGFR expression in Schwann cells.